Effects of plant growth regulators on the callus induction of banana leaves in Vitro

Using in vitro suckers of ‘Brazilian’ banana cultivar as materials,MS+6-BA 4.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+ agar 5.0 g/L as the shoot proliferation medium,effects of plant growth regulators on callus induction from Banana leaf and its proliferation were studied.The results showed that,main factor influencing callus induction from banana leaves was auxins,while cytokinin and gibberellin had little effects.2,4-D was the main auxin influencing the callus induction.2,4-D alone could successfully induced watersoaked-like callus,and the induction rate was 100%;when used at the concentration of 0.5~2.0 mg/L 2,4-D could make the callus to form white and compact callus during subcultures.When 2,4-D and NAA were both used in medium,the water-soaked-like callus could differentiate into light green granular callus.