Sterility Investigation and Genetical Analysis of a Thermosensitive Genic Male Sterile Mutant 0A15-1 in Rice

A somaclonal mutant 0A15-1 obtained from in vitro culture by young panicles of IR69700A showed typically abortive type of male sterility as proved by pollen staining with 1% iodide-potassium iodide solution. As shown by short day and low temperature treatments, the mutant had a character of thermosensitive genic male sterility which exhibited fertility conversation, i.e. sterile under high temperature and fertile under low temperature, regardless of being under long day or short day. Analysis of fertility segregation pattern from F1, F2 and BC1 indicated that the sterility trait of 0A15-1 was controlled by a single recessive gene and belonged to sporophytic male sterile type. This mutant is an useful material both for gene tagging and production of hybrid rice by means of photo(thermo)-sensitive male sterile lines.