2005•Unpublished venueRequires access
Gene cloning of maize sucrose phosphate synthetase and construction of plant expression vector
Baohui Liu
Open publisher page 0 citations
Abstract
By means of RT-PCR methods,we have cloned the all cDNA fragment of maize SPS gene from the total RNA in maize seedling leaf.These fragments have 99% expressing vector(PBISPS),respectively,using CaMV35S as primer,Tnos as plant binary expression vector of terminater;using pcab as primer,T35s as plant expression vector of terminater(PBSPS).Among them PBISPS contains NPT selective mark gene,PBSPS don't have.