The inhibitory effects of exogenous ING4 gene expression on the growth of breast cancer cells

Objective:To observe the inhibitory effects of ING4 gene expression on the growth of MCF-7 cells.Methods:ING4 mRNA expression in three breast cancer lines was detected by real-time fluorogentic quantitative-PCR(RFQ-PCR).pcDNA3.1(+)ING4 was transfected into MCF-7 cells.The proliferation activity of MCF-7 cells was measured by MTT assay and flow cytometry.Cell apoptotic ratios were analyzed by Annexin-V/PI double staining.Results:The expression of ING4 was markedly lower in MCF7,MDA-MB-435,and MDA-MB-231 cells compared with normal breast tissues.Over expression of ING4 slowed down proliferation speed of MCF-7 cells,increased the cell proportion in G1 phase and decreased the proportion in S phase(P0.05).The apoptotic ratio of MCF-7 cells was significantly increased compared to control group(P0.05).RT-PCR revealed that expressions of p21 and bax gene were all up-regulated but p53 had no change.Conclusion:ING4 was associated with tumorigenesis of breast cancer.Over expression of ING4 inhibited the proliferation of human breast cancer MCF-7 cells.