Effects of biomaterial with transforming growth factor-beta (TGF-beta)1.on regulating proliferation activity and receptor signal gene expression in mouse osteoblast-like MC3T3-E1 cells

Objective: To evaluate the effects of TGFβ1 on regulating the levels of proliferation activity and the mRNA transcriptions of TGF-beta1 receptor in mouse osteoblast-like cells. Methods: Mouse osteoblastic cell line MC3T3-E1 was selected as the effective cell of TGF-beta1. After the cells were treated.[3H]-Thymidine copration assays was used for examining the cell proliferation. RT-PCR employed for determining the levels of TGF-beta1 receptor mRNAs. Results: After the MC3T3-E1 cells were treated with TGF-beta1 at the dosages of 1 0 ng/mlfor 24h,[3H]-Thymidine copration assays increased markedly (P 0.05 or P 0.01). the levels of TGF-beta1 receptors mRNA increased significantly (P 0.01). Conclusions: TGF-beta1 at the dosages of 10ng/mL showed the effects on promoting proliferation,which probably leaded to the maintenance of high TGF-beta1 receptors levels. TGF-beta1 receptor I gene expression at the level of transcription was up regulated in the MC3T3-E1 cell treated with dosage of TGF-beta1 (10 ng/ml) and TGF-beta1 receptor Ⅱ mRNA was also,which might be one of the mechanisms for the maintenance of high TGF-beta1 receptor levels.TGF-beta1 (10 ng/ml) induce immediate early gene expression and growth in MC3T3-E1 osteoblasts primarily through pathway of TGF-beta1 receptors.