Comparison of Influenza Virus Titers Cultured in Vero and MDCK Cells

Objective To explore the feasibility of culture of influenza virus type A1 in Vero and MDCK cells and optimize the culture condition.Methods Inoculate influenza virus type A1 into Vero and MDCK cells and culture in media containing fetal bovine serum(FBS) or trypsin at different concentrations.Harvest the virus liquid at different time for determination of HA titers.Results FBS inhibited the proliferation of influenza virus significantly.The addition of about 5 μg/ml of trypsin increased the virus yield.The optimal time for harvesting virus was 72-96 h after cultivation.The HA titer of influenza virus in MDCK cells was significantly higher than that in Vero cells.Conclusion Both Vero and MDCK cells were suitable for culture of influenza virus and development of influenza vaccine.