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CLONING AND SEQUENCING OF GENE ENCODING THE RANA CATESBEIANA RIBONUCLEASE MATURE PROTEIN

Yan Liu

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Abstract

Objective:To obtain cDNA encoding Rana catesbeiana ribonuclease mature protein.Methods:The cDNA encoding Rana catesbeiana ribonuclease mature protein was amplified by RT PCR technique from the liver of the female Rana catesbeiana.The gene fragment was cloned into plasmid pUCm T,and identified by endonucleases digestion and sequencing.Results:Sequencing confirmed that the cDNA encoding Rana catesbeiana ribonuclease mature protein was correctly inserted in recombinants.Conclusion:Recombinant gene encoding Rana catesbeiana ribonuclease mature protein laid the foundation for further research on biological activities and antitumor effect of Rana catesbeiana ribonuclease.

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What this paper is about

Objective:To obtain cDNA encoding Rana catesbeiana ribonuclease mature protein.Methods:The cDNA encoding Rana catesbeiana ribonuclease mature protein was amplified by RT PCR technique from the liver of the female Rana catesbeiana.The gene fragment was cloned into plasmid pUCm T,and identified by endonucleases digestion and sequencing.Results:Sequencing confirmed that the cDNA encoding Rana catesbeiana ribonuclease mature protein was correctly inserted in recombinants.Conclusion:Recombinant gene encoding Rana catesbeiana ribonuclease mature protein laid the foundation for further research on biological activities and antitumor effect of Rana catesbeiana ribonuclease.

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Available abstract

Objective:To obtain cDNA encoding Rana catesbeiana ribonuclease mature protein.Methods:The cDNA encoding Rana catesbeiana ribonuclease mature protein was amplified by RT PCR technique from the liver of the female Rana catesbeiana.The gene fragment was cloned into plasmid pUCm T,and identified by endonucleases digestion and sequencing.Results:Sequencing confirmed that the cDNA encoding Rana catesbeiana ribonuclease mature protein was correctly inserted in recombinants.Conclusion:Recombinant gene encoding Rana catesbeiana ribonuclease mature protein laid the foundation for further research on biological activities and antitumor effect of Rana catesbeiana ribonuclease.

Key concepts: Ribonuclease, Complementary DNA, Biology, Gene, Cloning (programming), Rana, Molecular biology, RNase P

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