Screening of cellulase high-yield strains by mutant
Zhifei He
Abstract
Zhifei He
Abstract
In this research,a strain capable of producing cellulase-Trichoderma viride 13010 was applied,T.viride 13010 was mutated by synergistic mutation for increasing cellulase production.We got a high cellulase product strain named T.viride GL13010.The main results were as follows: Two mutation factors(UV,sodium nitrite) possessed mortal ratio trend,which positively related to the mutation factors' concentration.The cellulase production of mutation strain,named T.viride GL13010 could increase70.63% after repeated Synergistic mutation.It illuminated that complex mutation could weaken repellence and saturation by single mutation factor.
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In this research,a strain capable of producing cellulase-Trichoderma viride 13010 was applied,T.viride 13010 was mutated by synergistic mutation for increasing cellulase production.We got a high cellulase product strain named T.viride GL13010.The main results were as follows: Two mutation factors(UV,sodium nitrite) possessed mortal ratio trend,which positively related to the mutation factors' concentration.The cellulase production of mutation strain,named T.viride GL13010 could increase70.63% after repeated Synergistic mutation.It illuminated that complex mutation could weaken repellence and saturation by single mutation factor.
Key concepts: Cellulase, Trichoderma viride, Mutant, Mutation, Sodium nitrite, Strain (injury), Mutation breeding, Chemistry