THE ROLE OF MITOCHONDRIAL PATHWAY IN TANSHINONEIIA-INDUCED APOPTOSIS OF HeLa CELLS

Objective To explore the possible way of tanshinoneⅡ A-induced apoptosis of HeLa cells. Methods Human HeLa cells were cultured in vitro and treated with different concentrations of TanshinoneⅡA.The apoptosis of the cells was detected by flow cytometry.The mitochondria1 membrane potential was measured by flow cytometry after JC-1 staining.The release of cytochrome c(Cyto C) from mitochondrial was tested by Western blotting. Results As compared with the blank control,after 48-hour-action of different-concentration tanshinoneⅡA,the rate of apoptosis of HeLa cells and the expression of Cyto C in cytoplasm increased,and that of Cyto C in mitochondria decreased;the number of cells with higher mitochondria1 membrane potential(normal cells) decreased,and that with lower membrane potential increased,the difference being significant(F=116.5-1 064.6,q=4.11-75.38,P0.01). Conclusion TanshinoneⅡA involves in inducing apoptosis of HeLa cells is related to mitochondria membrane potential drop and its release of Cyto C.