Value of detecting MMP-9,ADA and TNF-α cerebrospinal fluid in diagnosis of tuberculous meningitis

OBJECTIVE To study the clinical value of detecting cerebrospinal fluid MMP-9,ADA and TNF-α in diagnosis of tuberculous meningitis.METHODS MMP-9 and TNF-α were detected by ELISA,ADA was detected by the end-point analysis,and the results were compared with control cases.RESULTS The levels of MMP-9,ADA and TNF-α in CSF of the tuberculous meningitis were(87.5±16.2)ng/ml,(15.0±6.9)U/L and(82.6±14.2)μg/L,their levels were(50.4±13.1)ng/ml,(4.7±2.1)U/L and(91.5±16.4)μg/L in purulent meningitis,their levels were(40.1±10.5)ng/ml,(3.9±1.4)U/L and(53.2±11.3)μg/L in viral meningitis.The levels of MMP-9 and ADA in the patients with tuberculous meningitis were significantly higher than that in other three groups.The level of MMP-9 in the patients with purulent meningitis and viral meningitis was significantly higher than that in control group,the level of TNF-αin tuberculous meningitis and purulent meningitis was significantly higher than that in viral meningitis,the level of TNF-α in viral meningitis was significantly higher than that in control group(P0.01).CONCLUSION The levels of CSF MMP-9,ADA and TNF-α have the certain value to assist to identify and diagnose the tuberculous meningitis.