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2011•Unpublished venueRequires access

Study on separation and purification of alkaline protease from B.licheniformis

XU Yun-jing

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Abstract

On the production from Bacillus licheniformis(B.licheniformis)of alkaline protease by ethanol precipitation,salt precipitation,DEAE anion exchange chromatography,gel filtration purification step 4.The enzyme activity as an index of the alkaline protease purification conditions were optimized.The results showed that:purified enzyme specific activity 62098U/mg,purification factor of 23.7,15.7% activity recovery.

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On the production from Bacillus licheniformis(B.licheniformis)of alkaline protease by ethanol precipitation,salt precipitation,DEAE anion exchange chromatography,gel filtration purification step 4.The enzyme activity as an index of the alkaline protease purification conditions were optimized.The results showed that:purified enzyme specific activity 62098U/mg,purification factor of 23.7,15.7% activity recovery.

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Available abstract

On the production from Bacillus licheniformis(B.licheniformis)of alkaline protease by ethanol precipitation,salt precipitation,DEAE anion exchange chromatography,gel filtration purification step 4.The enzyme activity as an index of the alkaline protease purification conditions were optimized.The results showed that:purified enzyme specific activity 62098U/mg,purification factor of 23.7,15.7% activity recovery.

Key concepts: Bacillus licheniformis, Alkaline protease, Chromatography, Protease, Chemistry, Size-exclusion chromatography, Ion exchange, Enzyme

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