Effect of the ERK Expression on Gastric Mucosal Cell by Immunity Histochemistry in Rats with Electro-Acupuncture at Stomach Meridian Acupoints

Objective:To observe the expression of the Extracelluar regulated protein kinase(ERK) immunity histochemistry on gastric mucosal cells in rats with electro-acupuncture at stomach meridian acupoints.Methods:72 rats were randomly divided into six groups,Water-immersion and restrained stress methods was adopted for stress-induced gastric mucosal injury rat model,gastric mucosal cells were separated by using pronase digestion method,and incubated by PD153035,a EGFR inhibitor and 100ml/L serum,ERK expression in gastric mucosal cells was tested by Immunity Histochemistry.Results:In comparison with those in the Normal group or Model group,the ERK expression in gastric mucosal cell in the Stomach meridian group and Gallbladder meridian group were significant reinforcement(P0.01).Furthermore,the ERK expression in Stomach meridian group were more obvious than those in Gallbladder meridian group(P0.01).Compared with Stomach meridian group,the reduction of the ERK expression in Stomach plus PD153035 group was more significant(P0.01).Conclusions:The electro-acupuncture can activate the ERK expression in gastric mucosal cells,ERK is one of the key material of the relative particularity between Stomach Meridian and Stomach.