Experimental research of Fuzheng xiaoliu decoction on inhibiting proliferation and inducting apoptosis of human hepatoma SMMC-7721 cells

Objective To investigate the effect of Fuzheng xiaoliu decoction on inhibiting proliferation and inducting apoptosis of human hepatoma SMMC-7721 cells.Methods€E? The influence of Fuzheng xiaoliu decoction on the proliferation of human hepatoma SMMC-7721 cells was determined by MTT assay.TNF-α production was determined by Elisa.The influence of Fuzheng xiaoliu decoction on apoptosis in human hepatoma SMMC-7721 cells was detected by flow cytometry.Immune staining method was used to determine bcl-2 and bax expressions.Results The results showed 100 μg/ml and 50 μg/ml dose groups could significantly inhibit the growth of human hepatoma SMMC-7721 cells from 12 hours to 84 hours.It could obviously promote tumor necrosis factor-α(TNF-α) generation of SMMC-7721 cells from 24 hours to 48 hours and apoptosis of SMMC-7721 cells,and the apoptosis rate increased with the concentration and time(24～72 h).High dose group had significant up-regulation effect of SMMC-7721 cells bax expression,and the effect was more obvious with the higher concentration.Both high and low-dose groups could significantly down-regulate bcl-2 expressions,and there was a statistical significance between high-dose group and model group(P 0.05).Conclusions The mechanism of Fuzheng xiaoliu decoction on tumor maybe related with promotingTNF-α generation,inhibiting tumor cell proliferation,inducing tumor cell apoptosis and regulating bax,bcl-2 expressions.