A Mice Mode of Chronic Intermittent Hypoxia with Carbon Dioxide Retention

Objective To establish a chronic intermittent hypoxia with carbon dioxide retention(CIH-CR)model in mice.Methods 22 male Kun Ming mice were divided into the normal control(NC) group and CIH-CR group.The mice of CIH-CR group suffered 8 hours intermittent hypoxia everyday for 4 weeks.The concentration of O2 and CO2in the cabin was detected by oxygen analyzer and Carbon dioxide detector,meanwhile,the blood oxygen saturation were detected by blood oximeter.At the end of the experiment,weight of right and left ventricles was measured.The general pathological changes of myocardium,pulmonary,kidney and brain were observed by HE staining.Result The concentration of O2 and CO2 in the cabin and the SO2 in the empennage of mouse accord with the cyclical change process.Compared with the NC group,the right ventricular hypertrophy were increased in the CIH-CR groups(P0.01).The tissue of myocardium,pulmonary,kidney and brain were showen obvious hypoxic change in CIH-CR group.Conclusion A CIH-CR animal model were be established successfully.