IGFBPrP1 siRNA induced apoptosis in rat hepatic stellate cells and its mechanism

Objective To investigate the effect of inhibiting insulin-like growth factor binding protein related protein1(IGFBPrP1) by chemically synthesized small interfering RNA(siRNA) on apoptosis of HSC-T6 cells and to explore the possible mechanism.Methods HSC-T6 were incubated in vitro and divided into three groups as following: normal control group,negative control group and IGFBPrP1 siRNA transfection group.The IGFBPrP1 siRNA was transfected into HSC-T6 cells.After transfecting with IGFBPrP1 siRNA at different time,cell proliferation was measured by CCK-8 kit and cell apoptosis was detected by flow cytometry with AnnexinV/PI double staining,both P53 and Bcl-2 protein expressions were detected by immunocytochemistry.Results(1) The inhibition ratio were decreased and the apoptotic rates of IGFBPrP1 siRNA transfection groups were increased compared with the normal control group and the negative control group at 24 h,48 h,72 h after transfection(P0.01);(2) Immunocytochemistry showed that the expression of P53 protein was significantly increased and the expression of Bcl-2 protein was significantly decreased compared with the normal control group and the negative control group at 48 h after transfection(P0.01).Conclusion IGFBPrP1 siRNA is able to inhibit the cell proliferation significantly and induce apoptosis of HSC-T6 cells effectively.Apoptosis of HSC-T6 cells induced by IGFBPrP1 siRNA may be associated with up-regulation of P53 expression and down-regulation of Bcl-2 expression.RNAi against IGFBPrP1 may provide a new strategy for the treatment of liver fibrosis.