ScholarLens Back to search
2005•Zhonghua zhongliu fangzhi zazhiRequires access

Inhibitory effects of shRNA targeting CD_(44)V_3 on proliferation ability of human lung carcinoma cell A549 in vitro

Zhengtang Chen

Open publisher page 0 citations

Abstract

OBJECTIVE:To investigate the effect of short hairpin RNA (shRNA) targeting CD_(44)V_3 on CD_(44)V_3 expression and proliferation ability of human lung carcinoma cell line A549 in vitro. METHODS: RNA interference plasmid that include U6 promoter and can express shRNA of CD_(44)V_3 was designed, constructed, and transfected into A549 cell line. CD_(44)V_3 expression was examined by reverse transcription polymerase chain reaction (RT-PCR) and western blot. Proliferation ability was examined by MTT assay and clone formation assay. RESULTS: After plasmid transfected, CD_(44)V_3 expression in A549 cell and proliferation ability of A549 cell decreased significantly. CONCLUSION: The shRNA targeting CD_(44)V_3 could inhibit efficiently proliferation ability of human lung carcinoma cell A549 in vitro.

About this research paper

What this paper is about

OBJECTIVE:To investigate the effect of short hairpin RNA (shRNA) targeting CD_(44)V_3 on CD_(44)V_3 expression and proliferation ability of human lung carcinoma cell line A549 in vitro. METHODS: RNA interference plasmid that include U6 promoter and can express shRNA of CD_(44)V_3 was designed, constructed, and transfected into A549 cell line. CD_(44)V_3 expression was examined by reverse transcription polymerase chain reaction (RT-PCR) and western blot. Proliferation ability was examined by MTT assay and clone formation assay. RESULTS: After plasmid transfected, CD_(44)V_3 expression in A549 cell and proliferation ability of A549 cell decreased significantly. CONCLUSION: The shRNA targeting CD_(44)V_3 could inhibit efficiently proliferation ability of human lung carcinoma cell A549 in vitro.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE:To investigate the effect of short hairpin RNA (shRNA) targeting CD_(44)V_3 on CD_(44)V_3 expression and proliferation ability of human lung carcinoma cell line A549 in vitro. METHODS: RNA interference plasmid that include U6 promoter and can express shRNA of CD_(44)V_3 was designed, constructed, and transfected into A549 cell line. CD_(44)V_3 expression was examined by reverse transcription polymerase chain reaction (RT-PCR) and western blot. Proliferation ability was examined by MTT assay and clone formation assay. RESULTS: After plasmid transfected, CD_(44)V_3 expression in A549 cell and proliferation ability of A549 cell decreased significantly. CONCLUSION: The shRNA targeting CD_(44)V_3 could inhibit efficiently proliferation ability of human lung carcinoma cell A549 in vitro.

Key concepts: Small hairpin RNA, A549 cell, Molecular biology, Cell growth, Transfection, Cell culture, MTT assay, RNA interference

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Inhibitory effects of shRNA targeting CD_(44)V_3 on proliferation ability of human lung carcinoma cell A549 in vitro — Research Paper | ScholarLens