Effect of trichinization on interferon-gamma,interleukin-4 and interleukin-5 of mice with allergic asthma

Objective To study the effect of trichinization on allergic asthma mice through observing the changes of interferon-gamma(IFN-γ),interleukin-4(IL-4)and interleukin-5(IL-5)levels in serum and bronchoalveolar lavage fluid(BALF)of mice with allergic asthma which were infected with Trichinella spiralis.Methods Fifteen BALB/c female mice were randomly divided into blank control group(group A),simple allergic asthma group(group B)and asthma followed by trichinization group(group C),five mice in each group.The model of simple allergic asthma and allergic asthma after trichinization were established.The serum and BALF of mice were taken and measured by enzyme linked immunosorbent assay(ELISA).The differences of IFN-γ,IL-4 and IL-5 were compared among the three groups.Results There were no significant difference of IFN-γ levels in serum and BALF between group A and group B(P0.05).But the IL-4 and IL-5 levels in group B were significantly higher than those in group A(P0.05).The IFN-γ level in group C was significantly higher than that in group A(P0.05),but there were no statistic difference of IL-4 and IL-5 levels between the two groups(P0.05).There were no significant difference of IFN-γ levels in serum and BALF between group C and group B(P0.05).But the IL-4 and IL-5 levels in group C were significantly lower than those in group B(P0.05).Conclusion Trichinization has inhibitory action on the expression of IL-4 and IL-5 in serum and BALF,but has no effect on IFN-γ of mice with allergic asthma.