Effects of chlorpromazine and verapamil on liver and kidney of rats with acute exposure to cadmium

Objective Chlorpromazine(CPZ) and Verapamil(Ver) were given to rats with acute exposure to Cd to study the effects of those on liver and kidney. Methods Four groups were used for the study.Three experimental groups were given subcutaneous injection of 25?μmol/kg CdCl 2,two of which were pretreated by administering ip injection of 12.5?mg/kg CPZ and 10?mg/kg Ver respectively one hour before the injection of CdCl 2.Control group was injected with 0.9%NaCl at the same time.Blood,liver and kidney were collected 24?h later after CdCl 2 administration.LDH,GPT activities in serum were determined.Cd,MDA and GSH contents in liver and renal cortex as well as GSH-Px activities were measured. Results In contrast with control group,LDH,GPT activities in serum and MDA,GSH contents in liver with Cd alone were increased significantly,and GSH-Px activities in both liver and renal cortex were obviously decreased.LDH activities in serum and MDA contents in liver with the administration of CPZ were decreased significantly compared with those of animals given Cd alone.GSH contents in renal cortex of CPZ-administrated group were significantly higher than those of control group.The group of Ver significantly reduced LDH,GPT activities in serum,GSH and MDA contents in liver.And it also increased obviosly GSH-Px activities in both liver and renal cortex compared with those with Cd. Conclusion CPZ and Ver protect rats against the toxic effects of Cd on liver to a degree,which may relate to reducing lipid peroxidation.CPZ and Ver may also affect the renal antioxidative system of rats with acute exposure to Cd.