Cloning and sequence characterization of cDNA encoding acetyl-CoA C-acetyltransferase in Camellia oleifera

Based on sequence information of acetyl-CoA C-acetyltransferase gene,six pairs of primers in conserved regions of this gene were designed.Total RNA of Xianglin No.1 seeds was used as template,one fragment of 718 bp was successfully amplified by 5'RACE strategy.Then the full cDNA of acetyl-CoA C-acetyltransferase gene was determined by assemblying the 5' sequence and known EST sequence that had been registered under GenBank(No.GU594059).The acetyl-CoA C-acetyltransferase gene of Camellia oleifera was 1 495 bp long,including a completed ORF of 1 227 bp,encoding 408 amino acids.On the amino acid sequence level,this gene showed the highest similarity(86%) with acetyl-CoA C-acetyltransferase gene of P.kurrooa,and the lowest(65%) with M.grisea.In addition,the predicted pI and Mw of Camellia oleifera acetyl-CoA C-acetyltransferase gene was 6.19,41 628.6 Da,respectively.