Effects of VEGF-siRNA on the growth of TCA8113 cell line of human tongue cancer in vitro and in vivo

Objective To access the influence of vector-based small interfering RNA(siRNA) targeting vascular endothelial growth factor(VEGF),on the growth of human tongue carcinoma cell line(TCA8113) in vitro and in vivo.Methods Two siRNA targeting VEGF constructed in eukaryotic expression vector(PU-VEGF-siRNA1,PU-VEGF-siRNA2),and vector without siRNA as experiment control,were transfected into TCA8113 cells by lipofectamine 2000,respectively.The non-transfected TCA8113 cell was used as negative control.The cell proliferation was detected by MTT.Twenty nude-mice were divided into four groups randomly,and then the transfected cells and non-transfected cell were subcutaneous injected on the back of mice,respectively.The tumor volume,weight,and growth curve of every group were compared.Results Compared with experiment and negative controls,cell proliferation was decreasend(P0.05),the growth inhabiting rate was risedn(P0.05) in vitro;the growth of tumors was significantly reduced,and tumor volume and weight decreased(P0.05),in two experiment groups.But there were no significant difference of those between two controls(P0.05).Conclusion VEGF-siRNA can suppress the TCA8113 growth effectively both in vitro and in vivo.