The Room Cultivation and Gene Transformation of Arabidopsis thaliana in Xinjiang Arid Region

Objective:Obtaining the robust plants of Arabidopsis thaliana and establishing a good transformation system of Arabidopsis in the special climate characteristics of Xinjiang arid region. Method:The cultivation conditions of Arabidopsis thaliana had been explored through some improvements on growing matrix, light period and relative humidity, and the foreign functional gene transformation on Arabidopsis thaliana was conducted by Agrobacterium. Result: The suitable cultivation conditions of Arabidopsis thaliana was the materials vermiculite: perlite (3∶1),as growing matrix, watering with 1/2 MS solution,the growth of Arabidopsis thaliana with 8h light in the former 4 weeks,16h light later,and 40%(60 % relative humidity, and efficiently genetic transformation system was established. Conclusion:It made a good foundation for scientific research by using Arabidopsis thaliana in Xinjiang.