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Inhibition Effect of AcSDKP on Unilateral Ureteral Obstruction-induced Renal Interstitial Fibrosis in Rats

Rujuan Xie

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Abstract

OBJECTIVE: To investigate the inhibition effect of Acetyl-Ser-Asp-Lys-Pro (AcSDKP) on unilateral ureteral obstruction (UUO)-induced renal interstitial fibrosis in rats and its mechanism. METHODS: 18 male rats were randomly divided into sham-operated group (normal saline), model group (normal saline) and treatment group (400 ?g·kg-1 AcSDKP). Those rats were given hypodermic injection twice a day for 1 day to establish UUO models. Rats were sacrificed 14 days later. Histological change of renal tissue was observed. The protein expression and mRNA expression of transforming growth factor β1 (TGF-β1) and vascular endothelial growth factor (VEGF) in renal tissues were detected. RESULTS: Compared with sham-operated group, model group and treatment group exhibited severe renal interstitial fibrosis and the level of TGF-β1 and its mRNA expression were enhanced (P0.05), while the protein and mRNA expression of VEGF were attenuated (P0.05). Compared with model group, tubular degeneration and renal interstitial fibrosis were improved (P0.05) and the level of TGF-β1 and its mRNA expression were attenuated (P0.05); the protein expression and mRNA expression of VEGF were enhanced significantly (P0.05) in treatment group. CONCLUSION: AcSDKP might attenuate the expression of TGF-β1 and enhance the expression of VEGF to inhibit renal interstitial fibrosis.

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OBJECTIVE: To investigate the inhibition effect of Acetyl-Ser-Asp-Lys-Pro (AcSDKP) on unilateral ureteral obstruction (UUO)-induced renal interstitial fibrosis in rats and its mechanism. METHODS: 18 male rats were randomly divided into sham-operated group (normal saline), model group (normal saline) and treatment group (400 ?g·kg-1 AcSDKP). Those rats were given hypodermic injection twice a day for 1 day to establish UUO models. Rats were sacrificed 14 days later. Histological change of renal tissue was observed. The protein expression and mRNA expression of transforming growth factor β1 (TGF-β1) and vascular endothelial growth factor (VEGF) in renal tissues were detected. RESULTS: Compared with sham-operated group, model group and treatment group exhibited severe renal interstitial fibrosis and the level of TGF-β1 and its mRNA expression were enhanced (P0.05), while the protein and mRNA expression of VEGF were attenuated (P0.05). Compared with model group, tubular degeneration and renal interstitial fibrosis were improved (P0.05) and the level of TGF-β1 and its mRNA expression were attenuated (P0.05); the protein expression and mRNA expression of VEGF were enhanced significantly (P0.05) in treatment group. CONCLUSION: AcSDKP might attenuate the expression of TGF-β1 and enhance the expression of VEGF to inhibit renal interstitial fibrosis.

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Available abstract

OBJECTIVE: To investigate the inhibition effect of Acetyl-Ser-Asp-Lys-Pro (AcSDKP) on unilateral ureteral obstruction (UUO)-induced renal interstitial fibrosis in rats and its mechanism. METHODS: 18 male rats were randomly divided into sham-operated group (normal saline), model group (normal saline) and treatment group (400 ?g·kg-1 AcSDKP). Those rats were given hypodermic injection twice a day for 1 day to establish UUO models. Rats were sacrificed 14 days later. Histological change of renal tissue was observed. The protein expression and mRNA expression of transforming growth factor β1 (TGF-β1) and vascular endothelial growth factor (VEGF) in renal tissues were detected. RESULTS: Compared with sham-operated group, model group and treatment group exhibited severe renal interstitial fibrosis and the level of TGF-β1 and its mRNA expression were enhanced (P0.05), while the protein and mRNA expression of VEGF were attenuated (P0.05). Compared with model group, tubular degeneration and renal interstitial fibrosis were improved (P0.05) and the level of TGF-β1 and its mRNA expression were attenuated (P0.05); the protein expression and mRNA expression of VEGF were enhanced significantly (P0.05) in treatment group. CONCLUSION: AcSDKP might attenuate the expression of TGF-β1 and enhance the expression of VEGF to inhibit renal interstitial fibrosis.

Key concepts: Medicine, Transforming growth factor, Endocrinology, Saline, Vascular endothelial growth factor, Internal medicine, Messenger RNA, Fibrosis

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Inhibition Effect of AcSDKP on Unilateral Ureteral Obstruction-induced Renal Interstitial Fibrosis in Rats — Research Paper | ScholarLens