Research of bone remodeling in osteoporosis rats with type 2 diabetes mellitus

Objective To characterize bone remodeling by establishing type 2 diabetic osteoporosis rat model.Methods Female Wistar rats were randomly divided into sham operation group(NS);bilateral ovariectomy group(NOVX);diabetic sham operation group(DS) and diabetic ovariectomized group(DOVX).Ovariectomy was performed in both control and diabetic rats after type 2 diabetic models had been made.Venous blood samples were collected at 0、4、 8 and 12wks after ovariectomy,fasting plasma glucose(FPG)、fasting insulin(FINS)、estrogen(E2) and specific bone turnover markers such as alkaline phosphatase(B-ALP),tartrate-resistant acid phosphatase(TRACP5b) were all measured.BMD were measured,osteoblast and osteoclast cell were isolated and cultured from rats of all groups.Results At 0wks,FPG had significantly increased when DOVX′ 13.1±4.9 mmol/L compared with NS′ 4.4±0.6 mmol/L(all P0.05).at 4wks,BMD had significantly decreased when DOVX′ 0.073±0.012 g/cm2 compared with DS′ 0.098±0.016 g/cm2(P0.05).at 4、8 and 12wks,there was no differences in plasma B-ALP levels when DOVX rats compared with NS rats.at 4 and 8wks,compared with NS rats,DOVX rats had significantly increased plasma TRACP5b concentration(all P0.05).For the activity of osteoblast,there wasno differences when DOVX compared with NS,NOVX and DS group.osteoclast numbers was significantly increased in DOVX rats than those in NS rats(P0.05).Conclusion There was active bone resorption and relatively slow bone formation in osteoporosis rats with type 2 diabetes mellitus.