Cloning and expression of FAM3B gene in gastric carcinoma cell line BGC-823

Objective To construct an eukaryotic expression vector of human FAM3B full-length gene, pEGFP-N2-FAM3B, transfect it into gastric cancer cell line BGC-823 and observe its effects on BGC-823, in a search for new approach of tumor therapy. Method The full-length gene sequence of FAM3B (708 bp) was obtained with RT-PCR using human gastric epithelium mRNA as the template. The PCR products were transferred into pEGFP-N2 vector using gene recombinant techniques. The plasmid of interest was detected by restrictive enzyme digestion and PCR, and then transfected into BGC-823 cells by lipid reagent. Results The pEGFP-N2-FAM3B eukaryotic expression vector was constructed successfully. The expression of green fluorescence protein (GFP) was observed in the human gastric cancer cell line BGC-823 after pEGFP-FAM3B transfection under fluorescence microscopy. Conclusion The eukaryotic expression vector pEGFP-N2-FAM3B was successfully constructed and expressed in BGC-823 cells.