Influence of Cryptotanshinone on Memory and Antioxidation Ability in APP/PS1 Double Transgenic Mice

OBJECTIVE To study the effect of cryptotanshinone on memory and antioxidation ability in APP/PS1 double transgenic mice.METHODS Three-month-old APP/PS1 double transgenic mice were randomly divided into 5 gorups.Besides,negative control mice were served as control group.There were 10 mice in each group and all of them were intragastrically administered for 4 months.Then the learning and memory ability of mice were detected by Morris water maze test.The activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD) and the content of malondialdehyde(MDA) in serum and brain tissue were measured with spectrophotometer.RESULTS Compared with control group,APP/PS1 double transgenic mice significantly prolonged the latency of searching the hidden platform in directional swimming test,decreased the time spent in swimming in the target quadrant in probe test.Compared with APP/PS1 double transgenic mice,cryptotanshinone could dose-dependently shorten the latency of searching the hidden platform in directional swimming test,increased the time spent in swimming in the target quadrant in probe test.The activities of GSH-Px and SOD decreased and the MDA content increased in APP/PS1 double transgenic mice,while in cryptotanshinone treatment groups,the activities of GSH-Px and SOD heightened and MDA content decreased,and there was significant difference compared with Alzheimer's disease model group.CONCLUSION Seven-month-old APP/PS1 double transgenic mice became imitated learning and memory dysfunction and the antioxidation ability reduction;cryptotanshinone could dose-dependently ameliorate the impaired learning and memory function of Alzheimer's disease mice and improve antioxidation ability.