Rat model of endotoxin sensitizing immature brain damage caused by hypoxia-ischemia

Aim:To study the effect of endotoxin(LPS) on the immature brain and to establish brain damage model of infection combined with hypoxia ischemia(HI) Methods: Seven day old rat pups were injected subclinical dose LPS or normal saline,then subjected to hypoxia ischemia with different hypoxia time after 4 h injection The brains were taken at 72 h post HI MAP 2 immunostaining was used Results:LPS at the dosage of 0 3 mg/kg had no side effect on the neonatal rat pups After combination of LPS and HI, brain damage was found in 20 min group,but not in 10 min group,while no marked brain damage in the control group Conclusion: Both LPS at a dosage of 0 3 mg/kg and HI 20 min could not produce brain damage alone The pronounced brain damage can be produced by the combination of LPS and HI It's suggested that LPS sensitize the immature brain to hypoxia ischemia damage