Preparation and Binding Characteristic of the Precursor of Chloramphenicol Imprinted Polymers

OBJECTIVE:To study the preparation method and the recognition properties of D-(-)-Threo-para-nitrophenyl-2-amino-1,3-propanediol(D-(-)-NAD)-precursor of chloramphenicol imprinted polymers.METHODS:The molecularly imprinted polymers were prepared with D-(-)-NAD as template molecules and with methylacrylic acid(MAA)as functional monomer.The formative mechanism of the imprinted polymers was evaluated by 1H NMR and ultraviolet-visible(UV)spectra.The binding characteristic and imprinting efficiency of polymers were investigated by using equilibrium binding experiments.RESULTS:The nitro-group and amino-group of the template can interact with functional monomer MAA through hydrogen bonding or ionic interaction in ethanol solution.The molecularly polymers prepared under this condition possess obvious specific recognition ability and stereo-selectivity.CONCLUSION:The polymer is expected to be further used as the high effective adsorbents or the chiral chromatographic stationary phase for the separation of D-(-)-NAD.