Influence of survivin gene repression by RNA interference on apoptosis and chemosensitivity of cervical cancer cell line Hela cells

AIM:To observe the effect of survivin gene repression by RNA interference(RNAi)on the apoptosis and the chemosensitivity of cervical cancer cell line Hela cells.METHODS:The small interfering RNA(siRNA)targeting against survivin was synthesized and transfected into Hela cells with lipofectamine 2000.Survivin expression was detected with the reverse transcription polymerase chain reaction(RT-PCR)and Western Blot.The level of proliferation was evaluated by drawing growth curve after cell counting.Flow cytometry was used to examine cell apoptosis,and the sensitivity to anticancer agents was evaluated by methyl thiazolyl tetrazolium(MTT)assay.RESULTS:Compared with the cells which were transfected with negative control siRNA(Hela-NC),pure lipofectamine(Hela-lip)and un-transfected cells(Hela),the expression level of survivin gene mRNA and protein declined evidently in two sets of cells transfected with survivin siRNA(siRNA 1 and siRNA 2),the expression inhibition rates were 55%,60% and 42%,55% respectively,the apoptosis rate was increased obviously(19% and 22%,P 0.05)and the level of proliferation were declined.At the same dose of anticancer agents,the sensitivity of cells in two interferential groups was increased,which was significant in NVB and PDD groups.CONCLUSION:Survivin siRNA can induce apoptosis by knocking down survivin gene expression and enhance chemosensitivity of Hela cells.