Study on tetramethylpyrazine protecting PC12 cell from apoptosis induced by diamide

Objective To analyze whether tetramethylpyrazine could protect PC12 cell from apoptosis induced by diamide in order to explore the mechanism of tetramethylpyrazine in the treatment of cerebral ischemia-reperfusion injury.Methods After PC12 cells were cultured by tetramethylpyrazine,diamide was added to induce the apoptosis of PC 12 cells.Various methods,including CCK-8 live cell test,Hoechst-33342 staining,flow cytometry,and quantitative reverse transcription/polymerase chain reaction(RT-PCR),were used to analyze the mechanisms involved in the process.Results After the pre-treatment,tetramethylpyrazine reduced the apoptosis of PC12 cells.The number of PC12 cells increased compared with decreased apoptosis rate.Tetramethylpyrazine could improve the activity of caspase 3,caspase 9 and the electric potential of mitochondrial membrane and the rate of Bax/Bcl-2 droped in the response to tetramethylpyrazine.Conclusions The pre-treatment with tetramethylpyrazine could reduce the apoptosis of PC12 cells damaged by diamide.