Effects of Ginkgo biloba Extract on Proliferation,Apoptosis and Caspase-3 Expression in Human Breast Cancer MCF-7 Cells

Objective:To investigate the effects of Ginkgo biloba extract(EGB) on proliferation,apoptosis and Caspase-3 expression in human breast cancer MCF-7 cells.Method:The human breast cancer MCF-7 cells cultivated in vitro were treated with 0,10,20,40,80,160 mg·L-1 of EGB,cultured for 24 h or 48 h.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT) assay was used to measure the cell proliferative effect.The annexin V/PI double staining analysis by flow cytometry was used to evaluate apoptotic rate and the cell cycle.The expression of Caspases-3 protein was detected by enzyme-linked immunosorbent assay(ELISA).Result:EGB could inhibit the proliferation of MCF-7 cells in vitro,and concentration-effect relationship was significant(P 0.01).50% inhibition concentration(IC50) of MCF-7 was 83.65 mg·L-1.The flow cytometry test indicated that EGB could make MCF-7 cells G0-G1 phase gradually increasing,G2-M period and S phase reduce gradually and with the increase of the concentration,MCF-7 cell apoptosis rate increased significantly(P 0.05 or P 0.01).EGB could enhance MCF-7 cells Caspases-3 protein expression(P 0.05 or P 0.01) and with the concentration dependent.Conclusion:EGB could effectively restrain human breast cancer MCF-7 cells Caspases-3 protein expression,induction MCF-7 cell apoptosis,inhibiting tumor cell proliferation.