ANTAGONISM OF CLOSTRIDIUM BUTYRICUM AND BIFIDOBACTERISM INFANT AGAINST CLOSTRIDIUM PERFRINGENS IN VITRO

This paper investigated antagonism of Bifidobacterism infantis LcL172 and Clostridium butyricum LcL166 against Clostridium perfringens NCTC8798 strain in vitro Either of LCL166 and LCL172 or both was mixed with proportionally C perfringens in GAM culture media respectively In the meantime C perfringens was incubated in GAM culture as a control During anaerobic incubation C perfringens was counted at intervals As a result,the multiplication of C perfringens was inhibited by either of LCL 172 or LCL166 or both of them significantly Both were able to restrain more multiplication of C perfringens than either of them It may be resulted from the inhibiting action of the organic acid produced by LCL172 and LCL166 by which lowed the growth of C perfringens was inhibited due to the decreased pH of the culture