Construction of eukaryotic expression vector for EGFR gene C-terminal domain

To construct the eukaryotic expression vector containing EGFR gene C-terminal domain.EGFR gene C-terminal domain from DH 5α is cloned by PCR,and subclons it into pcDNA 3.1(+) vector.The recombinant vector is vested by double-enzyme digestion and sequencing.The target fragment(1158 bp) is obtained as expected.pcDNA3.1(+)-hEGFR eukaryotic expression vector was successfully constructed.sequence analysis of the inserted target fragment revealed the same sequence as that published in Gen Bank.The pcDNA3.1(+)-hEGFR eukaryotic expression vector was successfully constructed.