Influence of Isoflovone on Learning and Memory and Monoamine Neuro-transmitter in Ovariectomized Rats Brain

Objective :To explore the effects and mechanisms of Isoflovone(IS)on spatial learning and memory and Monoamine Neuro-transmitter in ovariectomized rats hippocampus. Methods :Fifty young femal Sprague-Dawley rats were ovariectomized. Ovariectomized rats were randomly divided into five groups :control group,model group,estradiol benzoate(EB)group,IS low dose group,IS high dose group. All rats were evaluated for their spatial learning and memory performance with Morris watermaze task. The contents of Monoamine Neuro-transmitter and monoamine oxidase(MAO)in hippocampus were measured. Results :①As compared with model group,the latency period of control group and IS low dose group was decreased significantly(P0.05).②As compared with model group,contents of NE in hippocampus of control group,IS high dose group were increased significantly(P0.05). As compared with model group,contents of DA and 5-HT in hippocampus of control group,IS low dose group and EB group were increased significantly(P0.05).③As compared with model group,contents of MAO in hippocampus of control group,IS low dose group and EB group were increased significantly(P0.05). Conclusion :Isoflovone can improve the ovariectomized rats' impairment of spatial learning and memory by increasing contents of NE,DA,5-HT in hippocampus. Otherwise,isoflovone can reduce the contents of MAO in hippocampus.