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Study on replication deficient adenoviral vectors mediated enhanced green fluorescent protein transduction to neural stem cells

Boyong Mao

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Abstract

Objective:To study the feasibility and infection efficiency of replication deficient adenoviral vectors(AdVec) mediated enhanced green fluorescent protein(EGFP) transduction to neural stem cells(NSCs).Methods:Rat′s NSCs were cultured in vitro,AdVec-EGFP virus were amplified and purified.NSCs were transduced with AdVec-EGFP virus and results were estimated with fluorescence microscope and immnocytochemistry.Results:Report gene EGFP could be high performed in infected NSCs;fluorescein started to be expressed 6 hours after trasfected,and reached peak time afer 48 hours.The infection rate was(76±2)%;the biological characters of NSCs had no changes.Conclusions:AdVec-EGFP has higher gene expression efficiency of mediating exogenous gene;it is a better labelled compound for NSCs investigation both in vivo and in vitro.

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What this paper is about

Objective:To study the feasibility and infection efficiency of replication deficient adenoviral vectors(AdVec) mediated enhanced green fluorescent protein(EGFP) transduction to neural stem cells(NSCs).Methods:Rat′s NSCs were cultured in vitro,AdVec-EGFP virus were amplified and purified.NSCs were transduced with AdVec-EGFP virus and results were estimated with fluorescence microscope and immnocytochemistry.Results:Report gene EGFP could be high performed in infected NSCs;fluorescein started to be expressed 6 hours after trasfected,and reached peak time afer 48 hours.The infection rate was(76±2)%;the biological characters of NSCs had no changes.Conclusions:AdVec-EGFP has higher gene expression efficiency of mediating exogenous gene;it is a better labelled compound for NSCs investigation both in vivo and in vitro.

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Available abstract

Objective:To study the feasibility and infection efficiency of replication deficient adenoviral vectors(AdVec) mediated enhanced green fluorescent protein(EGFP) transduction to neural stem cells(NSCs).Methods:Rat′s NSCs were cultured in vitro,AdVec-EGFP virus were amplified and purified.NSCs were transduced with AdVec-EGFP virus and results were estimated with fluorescence microscope and immnocytochemistry.Results:Report gene EGFP could be high performed in infected NSCs;fluorescein started to be expressed 6 hours after trasfected,and reached peak time afer 48 hours.The infection rate was(76±2)%;the biological characters of NSCs had no changes.Conclusions:AdVec-EGFP has higher gene expression efficiency of mediating exogenous gene;it is a better labelled compound for NSCs investigation both in vivo and in vitro.

Key concepts: Green fluorescent protein, Transduction (biophysics), In vitro, Neural stem cell, Molecular biology, Biology, Fluorescence microscope, In vivo

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