Effect of Wnt3a on Pancreatic beta Cell Proliferation in Mice

Objective To analyze the effect of wnt3a on mice's pancreatic beta cell proliferation in vitro. Methods MIN6 cells with wnt3a protein were cultured in different concentrations and different time points,and the protein level was checked by Western blot.MIN6 cells with wnt3a were cultured and the cells were counted in 0,1,2,3,and 4 days.The number of cells was compared between wnt3a stimulating group and control group. Results Different concentrations of wnt3a had different effects on MIN6 cells, and the dilution of 1:8 was the best one to stimulate beta-catenin in MIN6 cells.Within different time points,6 hours was the best time point to stimulate beta-catenin in MIN6 cells.Compared the difference of control group and wnt3a group,we found that the cell proliferation in wnt3a group was significantly higher than that of control group. Conclusions wnt3a could promote the proliferation of pancreatic beta cells,which elucidates that wnt signal is important in pancreatic beta cell proliferation.