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Bacterial biofilm formed by haemophilus influenzae in vitro

You-ning Liu

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Abstract

Objective: To observe bacterial biofilm formed by Haemophilus Influenzae in vitro.Methods: 11 strains of Haemophilus Influenzae were isolated from sputum collected from patients with AECOPD.Formation of bacterial biofilm was examined by crystal violet assay and scanning electron microscope.Results: Striking differences were observed among strains with regard to the ability to form biofilm.Typical membrane-like structure formed by bacterial cells and extracellular matrix was detected.Conclusion: Haemophilus Influenzae is capable to form bacterial biofilm in vitro,which related with pathogenesis of AECOPD.

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What this paper is about

Objective: To observe bacterial biofilm formed by Haemophilus Influenzae in vitro.Methods: 11 strains of Haemophilus Influenzae were isolated from sputum collected from patients with AECOPD.Formation of bacterial biofilm was examined by crystal violet assay and scanning electron microscope.Results: Striking differences were observed among strains with regard to the ability to form biofilm.Typical membrane-like structure formed by bacterial cells and extracellular matrix was detected.Conclusion: Haemophilus Influenzae is capable to form bacterial biofilm in vitro,which related with pathogenesis of AECOPD.

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Available abstract

Objective: To observe bacterial biofilm formed by Haemophilus Influenzae in vitro.Methods: 11 strains of Haemophilus Influenzae were isolated from sputum collected from patients with AECOPD.Formation of bacterial biofilm was examined by crystal violet assay and scanning electron microscope.Results: Striking differences were observed among strains with regard to the ability to form biofilm.Typical membrane-like structure formed by bacterial cells and extracellular matrix was detected.Conclusion: Haemophilus Influenzae is capable to form bacterial biofilm in vitro,which related with pathogenesis of AECOPD.

Key concepts: Biofilm, Haemophilus influenzae, Microbiology, In vitro, Crystal violet, Haemophilus, Sputum, Bacterial cell structure

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