2012•Chinese Journal of Bone and Joint InjuryRequires access
An experimental research of composite of BMP-2 and PLGA/tricalcium phosphate with vascularized ulna or vascularized periosteum in repairing large segmental defect of sheeps' radius
Mi Zhou
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Abstract
Objective To study the effects of the composite of PLGA-TCP-BMP-2 artificial bone with vascularized ulna or vascularized periosteum in repairing the large segmental defect of sheep's radius.Methods Thirty millimeter defects were made in middle segments of sheep's radius and ulna.In group A,artificial bone with vascularized ulna were implanted into the radial defects.Artificial bone with vascularized periosteum were implanted into the radial defects in group B.Artificial bone were implanted into the radial defects in group C.No material was implanted in group D.Plates were used to fix the bone.X ray was used to evaluate the repairing effects.Animals were sacrificed 24 weeks after operation to make CT analysis and histological evaluation.Radiological and scoring histological systems were used to evaluate the plains.Results Twenty four weeks after surgery,radiological scoring systems revealed that group A was better than other groups.Evaluation of the morphologic features of the regenerating bone showed that active osteogenesis occurred at the site of bone defect and complete bony union was confirmed in animals of group A.In group B,corporation between new bone and fracture site could be seen.However,the lamellar bone was rather slim.In group C,new lamellar bone and lacuna were found in deranged alignment.In group D,deranged collagenous fibrous tissue,instead of osseointegration was found at the site of bone defect.Within the porous structure in animals of group A,B,and C,no residual artificial materials or muscle fibers were observed.According to histological scoring systems revealed that group A was better than other groups.Scores of group D was lower than other groups.Conclusion The composite of BMP-2 and PLGA/tricalcium phosphate with vascularized ulna or vascularized periosteum could repair large segmental defect of sheeps' radius satisfactorily.