Study on the HBV DNA presence from HBsAg-/NAT+/HBV DNA-donors screened by nucleic acid test

Objective To investigate the confirmation of HBV DNA positive from HBsAg/NAT + samples and study on the detection of HBsAg/ HBV DNA + samples. Methods From November 2011 to July 2012,the 81 out of 52 672 samples with HBsAgNAT +,but identification test-,were re-examined for the HBV DNA presence by both Nested-PCR and Real-time fluorescence quantitative detection. Results We obtained 15 HBsAg/ NAT + / HBV DNA + samples in 52 672 donors,of which 81 samples were HBsAg/ NAT + / HBV DNA-. After examined by Nested-PCR and QPCR,we finally confirmed 10 HBV DNA + samples in those previous 81 HBsAg/ NAT + / HBV DNAsamples; The detection rate of HBsAg/ HBV DNA + samples was 0. 05% [( 10 + 15 /52 672) ] and the viral load varied between undetection to 108. 9IU / mL. The viral load was more higher in anti-HBc + samples than anti-HBcsamples( P 0. 05). Conclusion The potential presence of false positive or false negative results in nucleic acid screening cannot be ignored,in order to guarantee the veracity of HBsAg/ HBV DNA + detection,it's important to improve both sensitivity and specificity in nucleic acid screening.