Construction and function identification of recombinant lentivirus vector expressing has-miR-146a

Objective To construct a lentivirus vector expressing microRNA (miRNA) miR-146a and transfect the vector into Jurkat cell line. Methods PrimiR-146a amplified by PCR was inserted into plenti-GFP vector,and then identified by restriction endonuclease digestion and nucleotide sequencing. Jurkat cell line was transfected with the plasmid pluG-miR-146a. The expression of miR-146a was detected by fluorescence microscopy,flow cytometry,and Real-time PCR. Results The sequence of miR-146a was contained in the constructed recombinant plasmid. After transfection with the plasmid,miR-146a could be expressed in Jurkat cell line. Conclusion The constructed lentivirus vector can express miR-146a in vitro,which is the basis for further study on the function of miR-146a.