Fermentation Conditions of Angiostatin by Recombinant E.coli

The fermentation conditions for production of recombinant human angiostatin (rhAGN) by E.coli M15 were carefully optimized. The optimal conditions were as follows:optimal temperatures was 37 ℃; initial pH was 7.0;the induction was started when OD_(600) reaching 0.8～1.0;IPTG concentration was 0.1 mmol/L, and the induction lasted for 6 h. The amount of the recombinant protein was 35% of the total cellular protein, and the concentration of rhAGN reached 560 mg/L.