Composition Analysis of High-molecular-weight Subunits of Glutenin of Wheat Hybridization Progenies

The compositions of high-molecular-weight(HMW) subunits of glutenin of the F2 progenies derived from Zhengzhou 9023×Yeereryan 4F were researched using sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE) method in the present study.The results showed that two,three and one HMW subunit types of glutenin were found in the loci of Glu-A1,Glu-B1 and Glu-D1,respectively,and three composition types appeared.For Glu-A1,subunits 1 and N occurred,and the frequency of high quality subunit 1 was as high as 66.7%;for Glu-B1,the frequency of subunit pair 13+16 was the highest(55.6%);for Glu-D1,only subunit pair 5+10(regarded as the highest quality subunit pair in the world) appeared.The frequency of composition type 1,13+16 and 5+10 was the highest(55.6%),which contained high quality subunit 1 and subunit pair 5+10.