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2011•Jiangsu Medical JournalRequires access

Protective effects of exogenous creatine phosphate pretreatment on myocardial ischemia-reperfusion injury in rats

Guoqiang Chen

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Abstract

Objective To study the protective effects of creatine phosphate pretreatment on myocardial ischemia-reperfusion(I-R) injury in rats.Methods Thirty adult male SD rats were randomly and equally divided into three groups of S(sham operated),I-R(ischemia for 30 min and reperfusion for 120 min) and CP(creatine phosphate 10 ml/kg intraperitoneally injected 30 min before ischemia).Myocardial tissues were tested for adenosine triphosphate(ATP) and superoxide dismutase(SOD) activity in the myocardium were examined.Myocardial ultrastructure and mitochondria swelling were observed under light and electron microscope.Results ATP level and SOD activity were higher in group CP than those in group I-R[(2.76±0.98) μmol/mg vs.(1.65±0.84) μmol/mg,(42.47±2.16) U/mg vs.(49.56±3.28) U/mg](P0.05).Compared to group I-R,the changes of myocardial ultrastructure and mitochondria in group CP were slighter.Conclusion The creatine phosphate pretreatment has the protective effects on the myocardium against I-R injury in rats.

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Objective To study the protective effects of creatine phosphate pretreatment on myocardial ischemia-reperfusion(I-R) injury in rats.Methods Thirty adult male SD rats were randomly and equally divided into three groups of S(sham operated),I-R(ischemia for 30 min and reperfusion for 120 min) and CP(creatine phosphate 10 ml/kg intraperitoneally injected 30 min before ischemia).Myocardial tissues were tested for adenosine triphosphate(ATP) and superoxide dismutase(SOD) activity in the myocardium were examined.Myocardial ultrastructure and mitochondria swelling were observed under light and electron microscope.Results ATP level and SOD activity were higher in group CP than those in group I-R[(2.76±0.98) μmol/mg vs.(1.65±0.84) μmol/mg,(42.47±2.16) U/mg vs.(49.56±3.28) U/mg](P0.05).Compared to group I-R,the changes of myocardial ultrastructure and mitochondria in group CP were slighter.Conclusion The creatine phosphate pretreatment has the protective effects on the myocardium against I-R injury in rats.

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Available abstract

Objective To study the protective effects of creatine phosphate pretreatment on myocardial ischemia-reperfusion(I-R) injury in rats.Methods Thirty adult male SD rats were randomly and equally divided into three groups of S(sham operated),I-R(ischemia for 30 min and reperfusion for 120 min) and CP(creatine phosphate 10 ml/kg intraperitoneally injected 30 min before ischemia).Myocardial tissues were tested for adenosine triphosphate(ATP) and superoxide dismutase(SOD) activity in the myocardium were examined.Myocardial ultrastructure and mitochondria swelling were observed under light and electron microscope.Results ATP level and SOD activity were higher in group CP than those in group I-R[(2.76±0.98) μmol/mg vs.(1.65±0.84) μmol/mg,(42.47±2.16) U/mg vs.(49.56±3.28) U/mg](P0.05).Compared to group I-R,the changes of myocardial ultrastructure and mitochondria in group CP were slighter.Conclusion The creatine phosphate pretreatment has the protective effects on the myocardium against I-R injury in rats.

Key concepts: Creatine, Ischemia, Adenosine triphosphate, Superoxide dismutase, Creatine kinase, Mitochondrion, Reperfusion injury, Phosphate

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