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2006•Journal of Microbiology and BiotechnologyRequires access

Proteomes induced by S-adenosyl-L-methionine in Streptomyces coelicolor A3(2)

Kwang Pyo Kim, Choonshik Shin, Soo-Jae Lee, Jihye Kim, Jungmo Young, Yukyung Lee, Ahn Joong-Hoon, Suh Joo-Won, Yoongho Lim

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Abstract

It was reported that an accumulation of S-adenosyl-L-methionine increases production of actinorhodin in Streptomyces lividans and induces antibiotic biosynthetic genes. We also obtained the same result in Streptomyces coelicolor A3(2). Therefore, in order to identify proteins changed by the addition of S-adenosyl-L-methionine in S. coelicolor A3(2), LC/MS/MS analyses were carried out. Thirteen proteins that were not observed in the control were found.

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What this paper is about

It was reported that an accumulation of S-adenosyl-L-methionine increases production of actinorhodin in Streptomyces lividans and induces antibiotic biosynthetic genes. We also obtained the same result in Streptomyces coelicolor A3(2). Therefore, in order to identify proteins changed by the addition of S-adenosyl-L-methionine in S. coelicolor A3(2), LC/MS/MS analyses were carried out. Thirteen proteins that were not observed in the control were found.

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Available abstract

It was reported that an accumulation of S-adenosyl-L-methionine increases production of actinorhodin in Streptomyces lividans and induces antibiotic biosynthetic genes. We also obtained the same result in Streptomyces coelicolor A3(2). Therefore, in order to identify proteins changed by the addition of S-adenosyl-L-methionine in S. coelicolor A3(2), LC/MS/MS analyses were carried out. Thirteen proteins that were not observed in the control were found.

Key concepts: Streptomyces coelicolor, Actinorhodin, Actinomycetales, Streptomycetaceae, Methionine, Streptomyces, Biochemistry, Proteome

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