Effect of target glucose control therapy on expression of tumor necrosis factor-α and endothelial cell specific molecule-1 for rats with sepsis

Objective: To explore the effect of target glucose control therapy on the expression of tumor necrosis factor-α( TNF-α) and endothelial cell specific molecule-1( ESM-1) and its effect on lung injury for rats with sepsis. Methods: Forty Sprague-Dawley rats were randomly divided into five groups: sham operation group( sham group),sepsis group( group CLP),glycemic control A group( 4. 4 ~6. 1 mmol/L),glycemic control B group( 6. 2 ~8. 3 mmol/L) and glycemic control C group( 8. 4 ~ 10. 0 mmol / L),eight rats for each group. The sepsis model of rat was established by cecal ligation and puncture( CLP),and the models were sacrificed 12 hours after CLP. Serum levels of TNF-α and ESM-1 were detected by enzyme-linked immunosorbent assay( ELISA),the pathological changes of lung tissue was observed by optical microscope. Results: The expressions of TNF-α and ESM-1 in sepsis groups were higher than those in sham group and all glycemic control groups( P 0. 05). They were significantly decreased in group A than those in group B and C( P 0. 05),and they were lower in group B than those in group C( P 0. 05). The pathology tissue injury score was significantly higher in sepsis group than those in sham group and all glycemic control groups( P 0. 05),and the lung pathology tissue injury score was significantly lower in group A( P 0. 05). Conclusion: Compared with glycemic control B group and C group,target glucose control management at 4. 6 ~ 6. 1 mmol / L can reduce the levls of TNF-α and ESM-1,and could significantly alleviate acute lung injury.