Antimicrobial Susceptibility and Resistance Mechanism of Pseudomonas Aeruginosa Resisting to Imipenem

Objective:To investigate the resistance and metallo-beta-lactamase of 44 strains of imipenem-resistant pseudomonas aeruginosa.Methods:Antimicrobial activity to 44 strains of pseudomonas aeruginosa were determined by K-B test,and the double-disk synergy test was used to screen metallo-beta-lactamase.PCR method was used to detect IMP gene and VIM gene.Results:The susceptibility rates to amikacin,ciprofloxacin,cefepime were 27.3%、20.5%、15.9%;respectively.Four among 44 imipenem-resistant Pseudomonas aeruginosa strains were positive for IMP gene,all 44 strains were negative for VIM gene.Conclusions:Amikacin,ciprofloxacin and cefepime will have better effect in treating the infection aroused by Pseudomonas aeruginosa resisting to imipenem.The production of IMP type metallo-beta-lactamase is the main mechanism for imipenem resistance of Pseudomonas aeruginosa in Beijing Tiantan Hospital.