Intravenous injection of mesenteric lymph from shock rats damages red blood cells in normal rats

AIM: To observe the effects of intravenous injection of the mesenteric lymph from shock rats on the characteristics and metabolism of red blood cells(RBC),and blood viscosity in normal rats.METHODS: The mesenteric lymph samples,collected from the rats 1 to 3 h after hemorrhagic shock,centrifuged to remove all cellular components and diluted with equal volume of saline,were intravenously injected into normal rats at dose of 2 mL/kg through femoral vein within 30 min.The equal volume of saline was intravenously injected into other normal rats as controls.At 2.5 h after injection,the blood samples were collected from the abdominal aorta for determining the routine parameters,adenosine triphosphate(ATP),lactic acid(LA),2,3-diphosphoglycerate(2,3-DPG),ion concentrations of intra-and extracellular fluid of the RBC and blood viscosity.RESULTS: Intravenous injection of shocked mesenteric lymph reduced the number of RBC,the concentration of hemoglobin,the hematocrit and the content of ATP.Intravenous injection of shocked mesenteric lymph significantly increased the mean corpuscular volume(MCV),2,3-DPG,LA in RBC and the whole blood reduced viscosity.However,no obvious effect of the injection on ion concentrations of intra-and extracellular fluid of RBC,whole blood viscosity and plasma viscosity was observed.CONCLUSION: Intravenous injection of shocked mesenteric lymph causes the disorders of energy metabolism in RBC,thus increasing the MCV and whole blood reduced viscosity.Shocked mesenteric lymph damages RBC.