Analyses of expression and function of P-glycoprotein in relapsed/refractory acute myelogenous leukemia patients

Objective To study the expression and function of P-glycoprotein in patients of relapsed/refractory acute myelogenous leukemia(AML). Methods CD34 expression was detected by immunohistochemistry, P-gp expression was detected by RT-PCR and P-gp function was analyzed by flow cytometry. Results CD34 expression has positive relevance with P-gp expression and function(r =0.621, P 0.001; r =0.496, P 0.001). P-gp expression has positive relevance with P-gp function (r = 0.574, P 0.01); positive rate of CD+34 in relapsed/refractory group (66.7 %) was higher than that in de novo group (32.6 %)(P 0.05). Positive rate of P-gp function in relapsed/refractory group (66.7 %) was higher than that in de novo group (25.6 %) (P 0.01). Conclusion CD34 expression has positive relevance with P-gp expression and function. Positive rate of CD34 and P-gp function in relapsed/refractory group are higher than those in De novo AML group. Monitoring P-gp function is more significant in clinical application.