Mechanism of TNF-α in Stimulation of Bone Marrow Mesenchymal Stem Cells in Rats

Objective:To investigate the promotive effects of NF-κB on the marrow-derived mesenchymal stem cells.Methods:Mononuclear cells were isolated from rat bone marrow by density-gradient centrifugation and were cultured on fibronectin-coated plates,supplied with bovine pituitary extract.MSCs were identified by Morphology and Surface molecule markers of MSCs.After the mar-row-derived mesenchymal stem cells were stimulated by TNF-α,the production of the growth factors and the protein expression of NF-κB in different groups were compared by ELISA and WESTERN BOLT.Results:① The marrow-derived mesenchymal stem cells were successful cultured by observing the morphology of the marrow-derived mesenchymal stem cells and detecting the cell surface antigen by flow cytometry assays.② The production of the growth factors had significantly increased in TNF-α stimulation group compared with that of no TNF-α stimulation group.The expression of p-IκB increased significantly indicating the activation of NF-κB(P0.05).The production of the growth factors had significantly decreased in stimulation of TNF-α plus the inhibitor of NF-κB group compared stimulation of TNF-α group,.And the protein expression of p-IκB significantly decressed indicate the inhibition of NF-κB(P0.05).Conclusion:NF-κB had crucially protective effects on the marrow-derived mesenchymal stem cells stimulated by TNF-α.