Surveillance of dengue vector and study of control ling Aedes albopictus in city area of Foshan
Jianwei Liu
Abstract
Jianwei Liu
Abstract
Objective To understand the c hange of density of dengue vector Aedes albopictus and the breeding habit , the distribution and the control effect of insecticides in city area of Foshan.T he result was to provide guidance for dengue control.Methods T he larva collected from different area and container was identified for species and the positive rate of larva was checked.The positive rate of larva was surve yed by artificial container with the same method. The man-alluring-biting metho d was used to survey the rate of biting. Reverse transciption-polymerase chain r eaction (RT-PCR) combined with virus isolation in C6/36 cell were detected dengu e virus in Aedes albopictus. The control effect of two insecticides for Ae des albopictus larva were observed in lab and mimic field. Results The Aedes albopictus density was higher in city area of Foshan . The Aedes albopictus breteau index(BI) was over 20% by artificial container and the biting rate index(BRI) was 1~22/per man power hour during all the year. The density of Aedes albopictus was equal to WHO three degree. The Aedes alb opictus adult density peak was in April-September and the larva density peak was from March to October.At all winter there were adult biting active and larva emerged. The change sea son density was related to the precipitation rain fall closely. The Aedes al bopictus larva distribution was extensive. The rate of positive in artificial container was more than that in nature. It bred in the tyre,jar,vat,bottle an d the small container form flower. Dengue virus were not detected in 40 pools of Aedes albopictus adult and in 60 pools of larva with RT-PCR combined with v irus isolation in C6/36 cell.It was indicated that two slow-release reagents wer e all effective for Aedes albopictus larva. Conclusion Ae des albopictus could survive all year in city area of Foshan. The density pea k was from April to September and was related to the precipitation rain fall clo sely. The Aedes albopictus population density had dangerous degree. The cont rol method should be used to tread small breeding container.Slow-release reagen ts may be effective for cointrolling Aedes albopictus larva.
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Objective To understand the c hange of density of dengue vector Aedes albopictus and the breeding habit , the distribution and the control effect of insecticides in city area of Foshan.T he result was to provide guidance for dengue control.Methods T he larva collected from different area and container was identified for species and the positive rate of larva was checked.The positive rate of larva was surve yed by artificial container with the same method. The man-alluring-biting metho d was used to survey the rate of biting. Reverse transciption-polymerase chain r eaction (RT-PCR) combined with virus isolation in C6/36 cell were detected dengu e virus in Aedes albopictus. The control effect of two insecticides for Ae des albopictus larva were observed in lab and mimic field. Results The Aedes albopictus density was higher in city area of Foshan . The Aedes albopictus breteau index(BI) was over 20% by artificial container and the biting rate index(BRI) was 1~22/per man power hour during all the year. The density of Aedes albopictus was equal to WHO three degree. The Aedes alb opictus adult density peak was in April-September and the larva density peak was from March to October.At all winter there were adult biting active and larva emerged. The change sea son density was related to the precipitation rain fall closely. The Aedes al bopictus larva distribution was extensive. The rate of positive in artificial container was more than that in nature. It bred in the tyre,jar,vat,bottle an d the small container form flower. Dengue virus were not detected in 40 pools of Aedes albopictus adult and in 60 pools of larva with RT-PCR combined with v irus isolation in C6/36 cell.It was indicated that two slow-release reagents wer e all effective for Aedes albopictus larva. Conclusion Ae des albopictus could survive all year in city area of Foshan. The density pea k was from April to September and was related to the precipitation rain fall clo sely. The Aedes albopictus population density had dangerous degree. The cont rol method should be used to tread small breeding container.Slow-release reagen ts may be effective for cointrolling Aedes albopictus larva.
Key concepts: Aedes albopictus, Larva, Biology, Aedes, Dengue fever, Vector (molecular biology), Aedes aegypti, Veterinary medicine