Determination on Inorganic Arsenic and Its Valence State in Urine of Patients with Acute Arsenic Poisoning

[Objective]Hydride Generation-Atomic Fluorescence Spectrometry was applied to determine the concentrations of inorganic arsenic,trivalent arsenic(As 3+)and pentavalent arsenic(As 5+).[Methods]6 mol/L HCl was used to extract the inorganic arsenic from poisoned patient's urine.The total concentration was determined under 2 mol/L HCl condition.The method of quantitative extraction of As3+ from inorganic arsenic solution into organic phase in strongly acidic conditions was adopted to extract As 3 + and As5 +.Finally,the concentrations were measured.[Results]Sample recovery rates of inorganic arsenic,As3 + and As5 + were 95.9%～103.5%,95.6%～101.6%,98.6%～103.1% respectively.The relative standard deviation of inorganic arsenic,As3 + and As5 + were 4.26%,6.68% and 5.38% respectively.[Conclusion]The method is suitable for determination of inorganic arsenic and its valence state in urine of patients with acute arsenic poisoning for its rapid analysis,high sensitivity,and less interference.